Emergency crews were called to Eastleigh Swan Centre after two customers struggled to breath when leaving a toilet.
Fire crews raced to the scene following the call just after 1.20pm.
After an assessment of the scene, it was discovered that a mix of chemicals in the drains had created a gas.
No one else was affected.
Joined by Ambulance teams, the pair were taking to Southampton General Hospital as a precaution.
A spokesman for the fire service said: “A couple of people who had used the toilet reported a bit of a smell and struggled to breath when leaving.
“We were called to investigate. It was discovered that it was just a couple of chemicals in the drains had mixed, producing a gas.”
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