Eastleigh travel to Vanarama Conference rivals Woking in the FA Trophy first round proper on 13 December.
In the other local draws, Weymouth will welcome Havant & Waterlooville and Wimborne Town face Oxford City.
In an all Conference South clash fifth place Basingstoke Town welcome third place Gosport Borough.
And Southern League Premier Division high fliers Poole Town will travel to either Maidenhead United if they beat Staines Town in their third qualifying round replay.
Here is the first round draw in full:
Local draws:
Woking VS Eastleigh
Weymouth VS Havant & Waterlooville
Wimborne Town VS Oxford City
Basingstoke Town VS Gosport Borough
Maidenhead United VS Staines T / Poole Town
Rest of draw:
Nuneaton Town VS Grimsby Town
Hyde VS Spennymoor Town
Altrincham VS Macclesfield Town
AFC Fylde VS Gainsborough Trinity
Guiseley / Rushall O VS Chorley / Stalybridge
Lincoln City VS Alfreton Town
North Ferriby United VS Boston United
Gateshead VS Halesowen Town
Worcester City VS FC Halifax Town
Southport VS Wrexham
Ramsbottom United VS Stockport County
Bradford (Park Avenue) VS Kidderminster Harriers
AFC Telford United VS Chester
Barwell / United VS Harrogate Town
Aldershot Town VS Burgess Hill Town
Bishop's Stortford VS Torquay United
Lowestoft Town VS Dover Athletic
Ebbsfleet United VS Welling United
St Alb City / Wealdstone VS Hayes & Yeading United
Bedford T / Weston SM VS Farnborough
Dartford VS Solihull Moors
Hemel Hempstead Town VS Sutton United
Tonbridge A / Bromley VS Leiston
Forest Green Rovers VS Merthyr / Didcot Town
Bristol Rovers VS Gloucester C / Bath City
Braintree Town VS St Neots T / AFC Sudbury
Concord Rangers VS Barnet
Comments: Our rules
We want our comments to be a lively and valuable part of our community - a place where readers can debate and engage with the most important local issues. The ability to comment on our stories is a privilege, not a right, however, and that privilege may be withdrawn if it is abused or misused.
Please report any comments that break our rules.
Read the rules here