A 16-year-old girl suffered "serious injuries" and remains in hospital in treatment after a double-decker bus crashed into the River Itchen.
Just before 10am yesterday, a Bluestar College 607 bus with 19 Barton Peveril College students on board smashed through railings on Bishopstoke Road and fell into the river.
The teenage girl was "seriously" injured, and the 69-year-old driver also remains in hospital with "serious injuries".
A further three passengers, two girls and one boy all 17, all sustained serious injuries and have since been discharged from hospital.
Some 15 other passengers aged between 16 and 18 suffered minor injuries, with three of these receiving hospital treatment.
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A heavy emergency services presence closed Bishopstoke Road yesterday but the road was reopened at 1am this morning, "following a complex recovery of the bus alongside the fire service", a spokesperson for Hampshire police said.
Detective Sergeant Gary Doughty, of the Serious Collision Investigation Unit, said: "This must have been a terrifying ordeal for everyone on the bus and for the families of the young passengers.
"We have worked closely with staff from the college to contact the families of all those involved and I want to thank the college for their assistance with this, as well as our ambulance and fire service colleagues for their help with the injured parties and recovery of the bus.
"Several members of the public helped passengers out of the bus before emergency services arrived and we have also had some initial witnesses who have given us key information to help us establish what happened.
"While we understand it will be tempting for people and the media to speculate on the potential causes of the collision, we just want to stress that officers are in the early stages of their investigation and are keeping an open mind.
"This will be a thorough investigation and all lines of enquiry will be followed to determine the circumstances."
Hampshire police are appealing for any witnesses or anyone with relevant dash cam footage to come forward.
A spokesperson for Hampshire police said: "You can call 101 quoting reference number 44250281666."
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