Gosport boss Mick Marsh watched his side slump to a 5-0 defeat at Andover and admitted: "The title's Eastleigh's."
Second-placed Gosport arrived in Andover a week after throwing away a two-goal lead at lowly Whitchurch.
But they came away from Andover with their Wessex League title hopes in tatters. Former Eastleigh man Vince Rusher marked his 250th game for Andover by helping his old Ten Acres teammates go a step closer to the championship.
But Rusher left the scoring to his Andover colleagues.
Danny Sullivan volleyed a left-wing cross past Geoff Sims after just three minutes and a Dean Cole brace ensured a 3-0 half-time lead before substitute Sam Rey and hotshot Justin Bennett added two more from close range.
Marsh said: "I think our chances went when we drew 2-2 with Whitchurch and, as far as I'm concerned, it's finished now.
"We're playing for second place, although I would have been happy with that at the start of the season.
"We're more than capable of winning all our remaining games and one of those is against Eastleigh, who still have to play Andover and Wimborne, but realistically it's over."
Gosport's 5-0 defeat equalled their biggest of the season - the 6-1 reverse at home to Eastleigh in October.
Marsh added: "It was a fair result, we didn't perform at all. We gave the ball away in midfield too much.
"Vince Rusher caused us lots of problems in a free role but we didn't defend well. I was surprised at how bad we were at this stage of the season and with so much to play for, but I take the blame because we kept trying to play football when the conditions weren't conducive to that. We should have been a bit more direct."
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