Alex McCarthy has pulled out of the England squad through injury, the Football Association has announced.
Tottenham left-back Danny Rose has also withdrawn due to a 'minor injury'.
Gareth Southgate's side travel a tough Nations League double-header this month, kicking off at Croatia on Friday before heading to Spain.
Rose and McCarthy missed the first training session as a precaution, but have now withdrawn from the squad.
"Having arrived in camp with minor injuries, @Alex_Macca23 and Danny Rose have now returned to their clubs and won't feature in this month's game," said the post on England's Twitter account.
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