Hampshire police were called to the incident on the northbound carriageway between junctions 13 and 12 to Eastleigh at 6.55am.
A car, van and HGV were involved in the collision, which did not result in any serious injuries.
Officers were moving the vehicles to the hard shoulder but the incident was causing significant tailbacks along the M3 and onto the M27 link road throughout the early morning rush hour.
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